Figure 1

Differential recovery of P. gingivalis W83 from different cell types on BAP at M.O.I. of 100. The cells (10⁵per well) were seeded in 24-well plates 24 hours prior to invasion. Log-phase P. gingivalis ( 10⁷) were resuspended in 1.0 ml antibiotic-free medium and added to each well. Three hours post invasion, the cells were lysed, P. gingivalis was recovered and CFU were enumerated. Data presented are average of four independent experiments, four plates per experiment. EC, primary endothelial cells; SMC, primary smooth muscle cells; KB, continuous epithelial cell line. Error bars denote standard deviation.