Mutational and transcriptional analyses of an avian pathogenic Escherichia coli ColV plasmid

BMC Microbiology

Table 3 Primers used in Mutagenesis*

Name	Primer Seq. (5'-3')	Target(s)*
for-mut-iutA	aataatgatgataagcaaaaagtatacgctttgggctctctgtgtaggctggagctgctt	iutA
rev-mut-iutA	atatcagcgtacctttgttgtaaaggaataccggtcagaacatatgaatatcctccttag	iutA, Vir2
for-mut-tsh	atactttatgtgcaggcataacggtgctctccctgaattttgtgtaggctggagctgctt	tsh, Vir1
rev-mut-tsh	ttcttctactgtaccgtaatcagataatcgcagcaaagggcatatgaatatcctccttag	tsh
for-mut-cvaB	cataagatatatcagccgggaggaaatgagccgatatttttgtgtaggctggagctgctt	cvaB
rev-mut-cvaB	tcatacgcttgtaattcctctatggtttaaatagaaataacatatgaatatcctccttag	cvaB
for-mut-sitA	gattgcatcattatttctccctttttccggctttaattcctgtgtaggctggagctgctt	sitA
rev-mut-sitA	tgctcactataggtactaaattatgcactcaataaaaaaacatatgaatatcctccttag	sitA
for-mut-iss	tattcatttcccatgattctgagtacctaccaagtctgagtgtgtaggctggagctgctt	iss
rev-mut-iss	aaaaacaactgtagggagcccagaagtatattaatgaacacatatgaatatcctccttag	iss
rev-mut-iroN	tattagggaataggtatgagaattaacaaaatcctctggtcatatgaatatcctccttag	iroN
for-mut-iroN	acgatcagaatgatgcggtaactccggcatagtaagcccgtgtgtaggctggagctgctt	iroN
for-mut-Vir2	tgctcactataggtactaaattatgcactcaataaaaaaatgtgtaggctggagctgctt	Vir2
rev-mut-Vir1	tattcatttcccatgattctgagtacctaccaagtctgagcatatgaatatcctccttag	Vir1

* Primers were created using information from GenBank Accession number NC007675.

ISSN: 1471-2180