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Table 2 Phenotypic Characteristics of Strains/Plasmids Used

From: Mutational and transcriptional analyses of an avian pathogenic Escherichia coli ColV plasmid

Strain or Plasmid

Relevant Characteristicsa

Reference and/or Source

APEC O2

Virulent to chick embryos; contains pAPEC-O2-ColV and pAPEC-O2-R

Diseased Chicken [1–3]

pAPEC-O2-ColV

ColV plasmid of APEC O2; contains iss, tsh, and traT genes, along with the aerobactin, ColV, sit and iro operons

[1,3]

pAPEC-O2-R

R plasmid of APEC O2; encodes resistance to multiple antimicrobial agents

[2,3]

NC

Relatively avirulent to chick embryos and lacks iss, tsh, and the aerobactin, ColV, and iro operons

Healthy Chicken [3]

NC/pAPEC-O2

Transconjugant derivative of NC containing pAPEC-O2-ColV and pAPEC-O2-R; has enhanced abilities to kill chick embryos and grow in human urine relative to strain NC

[3]

pKD46

ampR ; temperature sensitive plasmid which expresses the λ Red recombinase proteins when induced by arabinose

[30]

pSKY5000

camR derivative of pKD46

[42]

  1. a Abbreviations: Amp, ampicillin, Cam, chloramphenicol; ColV, colicin V; r, resistant