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Table 1 Elicitation of influenza A virus HA specific antibody in chickens immunized with HA expressing recombinant baculovirus.

From: WSSV ie1 promoter is more efficient than CMV promoter to express H5 hemagglutinin from influenza virus in baculovirus as a chicken vaccine

Inoculum

Dose

Route

HI GMT (range)

VN GMT (range)

ELISA GMT (range)

   

1st Dose

2nd Dose

1st Dose

2nd Dose

1st Dose

2nd Dose

vAc-G-ie-HA

109 PFU

IM

6.4

(4–8)

204.8a

(128–256)

32

(20–40)

192a

(160–320)

384

(320–640)

12288a

(10240–20480)

vAc-ie-HA

109 PFU

IM

3.2

(2–4)

11.2b

(8–16)

8

(<10–10)

28b

(20–40)

224

(160–320)

5120b

(5120)

vAc-G-CMV-HA

109 PFU

IM

2.4

(2–4)

76.8c

(64–128)

16

(10–20)

112c

(80–160)

288

(160–320)

9216c

(5120–10240)

vAc-CMV-HA

109 PFU

IM

1.6

(<2–2)

4.8d

(4–8)

6

(<10–10)

13d

(<10–20)

192

(160–320)

2560d

(2560)

H5N1/PR8 Inactivated

6 ug

IM

2

(2)

5.6

(4–8)

7

(<10–10)

22

(<10–40)

64

(40–80)

3072

(2560–5120)

H5N1/PR8

6 ug or 105 TCID50

IM

96

(32–128)

1024

(1024)

64

(40–80)

352

(160–640)

2560

(2560)

>20480

(20480)

PBS

100 ul

IM

1

(<2)

1

(<2)

5

(<10)

5

(<10)

5

(<10)

5

(<10)

  1. HI: hemagglutination inhibition titer of serum from immunized chickens. VN: virus microneutralization titer of serum from immunized chickens. GMT: geometric mean titer. GMT is calculated based on 5 different sera from 5 different chickens in each groups. For titers <2 in HI, 1 was used to calculate the GMT. A GMT of 1 was considered negative. For titer <10 in VN and ELISA, 5 was used to calculate the GMT. A GMT of 5 was considered negative. 1st Dose: serum samples collected at 2 weeks from the first inoculation. 2nd Dose: serum samples collected at 2 weeks from the second inoculation. IM: intramuscular.
  2. a-d p < 0.05. Values denoted by different letters are statistically different from each other in the same comparison, as analyzed in ANOVA test (not adjusted).