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Table 1 Elicitation of influenza A virus HA specific antibody in chickens immunized with HA expressing recombinant baculovirus.

From: WSSV ie1 promoter is more efficient than CMV promoter to express H5 hemagglutinin from influenza virus in baculovirus as a chicken vaccine

Inoculum Dose Route HI GMT (range) VN GMT (range) ELISA GMT (range)
    1st Dose 2nd Dose 1st Dose 2nd Dose 1st Dose 2nd Dose
vAc-G-ie-HA 109 PFU IM 6.4 (4–8) 204.8a (128–256) 32 (20–40) 192a (160–320) 384 (320–640) 12288a (10240–20480)
vAc-ie-HA 109 PFU IM 3.2 (2–4) 11.2b (8–16) 8 (<10–10) 28b (20–40) 224 (160–320) 5120b (5120)
vAc-G-CMV-HA 109 PFU IM 2.4 (2–4) 76.8c (64–128) 16 (10–20) 112c (80–160) 288 (160–320) 9216c (5120–10240)
vAc-CMV-HA 109 PFU IM 1.6 (<2–2) 4.8d (4–8) 6 (<10–10) 13d (<10–20) 192 (160–320) 2560d (2560)
H5N1/PR8 Inactivated 6 ug IM 2 (2) 5.6 (4–8) 7 (<10–10) 22 (<10–40) 64 (40–80) 3072 (2560–5120)
H5N1/PR8 6 ug or 105 TCID50 IM 96 (32–128) 1024 (1024) 64 (40–80) 352 (160–640) 2560 (2560) >20480 (20480)
PBS 100 ul IM 1 (<2) 1 (<2) 5 (<10) 5 (<10) 5 (<10) 5 (<10)
  1. HI: hemagglutination inhibition titer of serum from immunized chickens. VN: virus microneutralization titer of serum from immunized chickens. GMT: geometric mean titer. GMT is calculated based on 5 different sera from 5 different chickens in each groups. For titers <2 in HI, 1 was used to calculate the GMT. A GMT of 1 was considered negative. For titer <10 in VN and ELISA, 5 was used to calculate the GMT. A GMT of 5 was considered negative. 1st Dose: serum samples collected at 2 weeks from the first inoculation. 2nd Dose: serum samples collected at 2 weeks from the second inoculation. IM: intramuscular.
  2. a-d p < 0.05. Values denoted by different letters are statistically different from each other in the same comparison, as analyzed in ANOVA test (not adjusted).