Skip to main content

Advertisement

Table 1 Elicitation of influenza A virus HA specific antibody in chickens immunized with HA expressing recombinant baculovirus.

From: WSSV ie1 promoter is more efficient than CMV promoter to express H5 hemagglutinin from influenza virus in baculovirus as a chicken vaccine

Inoculum Dose Route HI GMT (range) VN GMT (range) ELISA GMT (range)
    1st Dose 2nd Dose 1st Dose 2nd Dose 1st Dose 2nd Dose
vAc-G-ie-HA 109 PFU IM 6.4
(4–8)
204.8a
(128–256)
32
(20–40)
192a
(160–320)
384
(320–640)
12288a
(10240–20480)
vAc-ie-HA 109 PFU IM 3.2
(2–4)
11.2b
(8–16)
8
(<10–10)
28b
(20–40)
224
(160–320)
5120b
(5120)
vAc-G-CMV-HA 109 PFU IM 2.4
(2–4)
76.8c
(64–128)
16
(10–20)
112c
(80–160)
288
(160–320)
9216c
(5120–10240)
vAc-CMV-HA 109 PFU IM 1.6
(<2–2)
4.8d
(4–8)
6
(<10–10)
13d
(<10–20)
192
(160–320)
2560d
(2560)
H5N1/PR8 Inactivated 6 ug IM 2
(2)
5.6
(4–8)
7
(<10–10)
22
(<10–40)
64
(40–80)
3072
(2560–5120)
H5N1/PR8 6 ug or 105 TCID50 IM 96
(32–128)
1024
(1024)
64
(40–80)
352
(160–640)
2560
(2560)
>20480
(20480)
PBS 100 ul IM 1
(<2)
1
(<2)
5
(<10)
5
(<10)
5
(<10)
5
(<10)
  1. HI: hemagglutination inhibition titer of serum from immunized chickens. VN: virus microneutralization titer of serum from immunized chickens. GMT: geometric mean titer. GMT is calculated based on 5 different sera from 5 different chickens in each groups. For titers <2 in HI, 1 was used to calculate the GMT. A GMT of 1 was considered negative. For titer <10 in VN and ELISA, 5 was used to calculate the GMT. A GMT of 5 was considered negative. 1st Dose: serum samples collected at 2 weeks from the first inoculation. 2nd Dose: serum samples collected at 2 weeks from the second inoculation. IM: intramuscular.
  2. a-d p < 0.05. Values denoted by different letters are statistically different from each other in the same comparison, as analyzed in ANOVA test (not adjusted).