Figure 4
From: Universal ligation-detection-reaction microarray applied for compost microbes
Probe specificity. a. The intensity values of all the probes against individual templates. On the Y-axis, the set of probes present in each reaction. On the X-axis, the template present in a given reaction. The colour coded values are intensity log ratios of LDR/B3. Highest values are expected to be in the diagonal. Three probes, PenCom-A50, KlyMa2-A84 and PichFerm-A67, do not detect any template. Probe AspFum-A58 is slightly non-specific to I. orientalis template. Pezizomycota-A29 is group-specific. b. Scanned images of an example slide with hybridisations of the complementary control probe and two probes specific for Thermomyces lanuginosus phylotype (TherLa in Fig. 4a) The control probe can be seen to hybridise to all of the spots and the specific probe for the corresponding zip-code sequences.