Figure 2From: The Vacuolar ATPase from Entamoeba histolytica: Molecular cloning of the gene encoding for the B subunit and subcellular localization of the proteinAlignment of polypeptide sequences of subunits A and B of the EhV-ATPase. Alignment was performed using the ClustalW program. Identical amino acids are shown in black boxes. Conserved changes are shown in gray boxes. Numbers correspond to amino acid positions in each polypeptide. Arrows indicate primers Bvac-S-373 and Bvac-AS-833 used to amplify by PCR a 465 bp DNA fragment containing a 154 aa region, which is a EhV-ATPaseB specific region.Back to article page