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Table 1 Clinical isolates and identification results from MicroScan, VITEK2, and Crystal GP

From: Comparison of the MicroScan, VITEK 2, and Crystal GP with 16S rRNA sequencing and MicroSeq 500 v2.0 analysis for coagulase-negative Staphylococci

Clinical Isolates (using MicroSeq 500)

No of isolates

Correct Identificationa

Low-level Discriminationb

Misidentificationc

Nonidentificationd

  

MicroScan

S. capitis

16

13 (81.3%)

-

3 (18.8%)

-

S. caprae

4

-

-

4 (100.0%)

-

S. cohnii

3

1 (33.3%)

1 (33.3%)

1 (33.3%)

-

S. epidermidis

53

51 (96.2%)

-

2 (3.8%)

-

S. haemolyticus

4

3 (75.0%)

1 (25.0%)

-

-

S. hominis

25

23 (92.0%)

2 (8.0%)

-

-

S. lugdunensis

6

2 (33.3%)

1 (16.7%)

3 (50.0%)

-

S. saprophyticus

1

1 (100.0%)

-

-

-

S. simulans

2

2 (100.0%)

-

-

-

S. warneri

6

3 (50.0%)

3 (50.0%)

-

-

Subtotal

120

99 (82.5%)

8 (6.7%)

13 (10.8%)

-

  

VITEK 2

S. capitis

16

16 (100.0%)

-

-

-

S. caprae

4

3 (75.0%)

-

1 (25.0%)

-

S. cohnii

3

3 (100.0%)

-

-

-

S. epidermidis

53

47 (88.7%)

6 (11.3%)

-

-

S. haemolyticus

4

4 (100.0%)

-

-

-

S. hominis

25

22 (88.0%)

1 (4.0%)

2 (8.0%)

-

S. lugdunensis

6

5 (83.3%)

1 (16.7%)

-

-

S. saprophyticus

1

1 (100.0%)

-

-

-

S. simulans

2

1 (50.0%)

1 (50.0%)

-

-

S. warneri

6

3 (50.0%)

-

3 (50.0%)

-

Subtotal

120

105 (87.5%)

9 (7.5%)

6 (5.0%)

-

  

Crystal GP

S. capitis

16

13 (81.3%)

-

3 (18.8%)

-

S. caprae

4

-

-

4 (100.0%)

-

S. cohnii

3

3 (100.0%)

-

-

-

S. epidermidis

53

49 (92.5%)

-

3 (5.7%)

1 (1.9%)

S. haemolyticus

4

4 (100.0%)

-

-

-

S. hominis

25

1 (4.0%)

-

15 (60.0%)

9 (36.0%)

S. lugdunensis

6

2 (33.3%)

-

3 (50.0%)

1 (16.7%)

S. saprophyticus

1

1 (100.0%)

-

-

-

S. simulans

2

2 (100.0%)

-

-

-

S. warneri

6

6 (100.0%)

-

-

-

Subtotal

120

81 (67.5%)

-

28 (23.3%)

11 (9.2%)

  1. Correct identificationa: single, unambiguous, correct identification at the species level
  2. Low-level discriminationb: two or more possible species level identification including the correct one
  3. Misidentificationc: genus or species-level identification different from that obtained with the reference method
  4. Nonidentificationd: no identification or unidentification