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Table 1 Primers and amplification conditions for the different PCR based screening of genes that encode for antibiotics

From: Assessment of genetic and functional diversity of phosphate solubilizing fluorescent pseudomonads isolated from rhizospheric soil

Gene and primer set Primer sequence Reference Amplification conditions
PCA (C, D)
PCA2a
PCA3b
5'-TGCCAAGCCTCGCT CCAAC-3'
5'-CGCGTTGTTCCTCGTTCAT-3'
77 Initial denaturation 94°C for 3 min, 30 cycles of 94°C for 60 s, 58°C for 45 s, and 72°C for 60 s. Final extension at 72°C for 10 min.
PCA (X, Y)
30–84/1
30–84/2
5'-CAGTTCATCCGGCGGGCTGCAG-3'
5'-CCCGTTTCAGTAAGTCTTCCATG-
ATGCG-3'
77 Initial denaturation 94°C for 3 min, 30 cycles of 94°C for 60 s, 58°C for 45 s, and 72°C for 60 s, Final extension at 72°C for 10 min.
PCN
PhzH-up
PhzH-low
5'-CGCACGGATCCTTTCAGAATGT-
TC-3'
5'-GCCACGCCAAGCTTCACGCTCA-3'
78 Initial denaturation 94°C for 30 s, 30 cycles of 94°C for 30 s, 64°C for 30 s, and 72°C for 7 min. Final extension at 72°C for 10 min.
DAPG
Phl2a
Phl2b
5'-GAGGACGTCGAAGACCACCA-3'
5'-ACC GCAGCATCGTGTATGAG-3'
79 Initial denaturation 94°C for 90 s, 35 cycles of 94°C for 35 s, 53°C for 30 s, and 72°C for 45 s. Final extension at 72°C for 10 min.
PLT
PltBf
PltBr
5'-CGGAGCATGGACCCCCAGC-3'
5'-GTGCCCGATATTGGTCTTGACCG-
AG-3'
79 Initial denaturation 94°C for 2 min, 29 cycles of 94°C for 1 min, 58°C for 45 s, and 72°C for 1 min, Final extension at 72°C for 10 min.
PRN
Prncf
Prncr
5'-CCACAA GCCCGGCCAGGAGC-3'
5'-GAGAAGAGCGGGTCGATGAAG-
CC-3'
79 Initial denaturation 94°C for 2 min, 30 cycles of 94°C for 1 min, 58°C for 45 s, and 72°C for 1 min. Final extension at72°C for 10 min.