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Table 1 Primers and amplification conditions for the different PCR based screening of genes that encode for antibiotics

From: Assessment of genetic and functional diversity of phosphate solubilizing fluorescent pseudomonads isolated from rhizospheric soil

Gene and primer set Primer sequence Reference Amplification conditions
PCA (C, D) PCA2a PCA3b 5'-TGCCAAGCCTCGCT CCAAC-3' 5'-CGCGTTGTTCCTCGTTCAT-3' 77 Initial denaturation 94°C for 3 min, 30 cycles of 94°C for 60 s, 58°C for 45 s, and 72°C for 60 s. Final extension at 72°C for 10 min.
PCA (X, Y) 30–84/1 30–84/2 5'-CAGTTCATCCGGCGGGCTGCAG-3' 5'-CCCGTTTCAGTAAGTCTTCCATG- ATGCG-3' 77 Initial denaturation 94°C for 3 min, 30 cycles of 94°C for 60 s, 58°C for 45 s, and 72°C for 60 s, Final extension at 72°C for 10 min.
PCN PhzH-up PhzH-low 5'-CGCACGGATCCTTTCAGAATGT- TC-3' 5'-GCCACGCCAAGCTTCACGCTCA-3' 78 Initial denaturation 94°C for 30 s, 30 cycles of 94°C for 30 s, 64°C for 30 s, and 72°C for 7 min. Final extension at 72°C for 10 min.
DAPG Phl2a Phl2b 5'-GAGGACGTCGAAGACCACCA-3' 5'-ACC GCAGCATCGTGTATGAG-3' 79 Initial denaturation 94°C for 90 s, 35 cycles of 94°C for 35 s, 53°C for 30 s, and 72°C for 45 s. Final extension at 72°C for 10 min.
PLT PltBf PltBr 5'-CGGAGCATGGACCCCCAGC-3' 5'-GTGCCCGATATTGGTCTTGACCG- AG-3' 79 Initial denaturation 94°C for 2 min, 29 cycles of 94°C for 1 min, 58°C for 45 s, and 72°C for 1 min, Final extension at 72°C for 10 min.
PRN Prncf Prncr 5'-CCACAA GCCCGGCCAGGAGC-3' 5'-GAGAAGAGCGGGTCGATGAAG- CC-3' 79 Initial denaturation 94°C for 2 min, 30 cycles of 94°C for 1 min, 58°C for 45 s, and 72°C for 1 min. Final extension at72°C for 10 min.