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Table 1 Predicted target specificties for ε34 DNA binding proteins

From: Genomic analysis of bacteriophage ε34 of Salmonella entericaserovar Anatum (15+)

  Predicted specificity1
DNA interaction P22 gene P22 ε34 ST64T ST104 Para-1 Scho-1
DNA packaging 3 P22 P22(96%) P22(96%) P22(96%) P22(98%) P22(99%)
Integration2 int P22(98%) thrW ε 34 argU P22(98%) thrW P22(98%) thrW P22(98%) thrW P22(98%) thrW
Early anti-termination 24 P22 ε 34,3 P22(92%) P22(98%) P22(96%) P22(93%)
Prophage repressor c2 P22 ε 34 L(100%) ST104 ST104 (100%) Scho1
DNA replication 18 5 P22 λ (94%)5,7 ST64T4 HK97 (98%)7 ST64T (97%)4 λ (94%)5,7
Late anti-termination 23 λ (96%)6 λ (99%) Sf6(97%)7 ST104 λ (99%) Sf6(96%)
  1. 1. On each line, the table indicates (without parentheses) the experimentally-determined specificty according to the prototypical phage with that specificty. If two proteins have ≥ 92% amino acid sequence identity, the specificty is predicted to be the same, and the percent amino acid sequence identity to the prototypical protein is given in parentheses. If two unstudied proteins have <92% amino acid sequence identity their specificity is predicted to be different and one was arbitrarily chosen to be the prototype (e.g., ST64T DNA replication).
  2. 2. The tRNA gene into which phage integrates is shown below the int protein comparisons.
  3. 3. Has 72% overall amino acid sequence identity to its phage HK97 homologue, but amino acids 74–113 are very similar to the HK97 protein (see text).
  4. 4. Both have 82% amino acid sequence identity to its phage Sf6 homologue; it is not known if proteins with this level of difference could have the same target specificity.
  5. 5. N-terminal domain only (amino acids 1–162). The whole ε34 and Scho1 putative replication origin recognition proteins (ε34 gp50 and Scho1 gpSC0340, respectively) are 96% and 97% identical to the phage ES18 homologue [33]. Their N-terminal regions are 94% identical to phage λ gpO amino acids 17 through 148, and the N-terminal 162 amino acids have been shown to bind the origin [81].
  6. 6. Shown experimentally to have the same specificity as λ gpQ [82].
  7. 7. Phages Sf6, HK97 and λ do not infect Salmonella, but the indicated Salmonella phage protein is predicted to have the same specificity as one of these phages.