Comparison of the levels of hugZ expression under different iron level conditions, detected by real-time quantitative RT-PCR. The results are based on the ratio hugZ mRNA amplification/gyrB mRNA amplification, which are presented as the fold induction of mRNA expression relative to the amount present in BBF. Real-time PCR was conducted in duplicate for each sample and the mean value was calculated. Expression values were calculated from three biological replicates. (BBF: Brucella Broth with 10% fetal bovine serum; BBF+Fe: BBF plus 50 μM FeCl3; BBF+Desferal: BBF plus 75 μM Desferal).