Figure 4

Separation of the solubilized membrane fractions from C. glutamicum ATCC 13032 in control condition (upper panel), versus acidic and basic conditions (left and right lower panels, respectively). Before separation in the first dimension by AIEC, the membranes were washed twice with 2.5 M NaBr and solubilized in buffer containing 2% (w/v) ASB-14. After the TCA precipitation of the AIEC fractions, SDS-PAGE was used as second dimension in Maxi size gels. Fraction numbers of the AIEC are given at the top of the gels. Numbers indicate the protein spots with differences among the 3 pH values chosen for MALDI-TOF analysis and are listed in Table 1. Molecular masses (in kDa) are shown on the left side of the panels.