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Figure 3 | BMC Microbiology

Figure 3

From: Response of the cytoplasmic and membrane proteome of Corynebacterium glutamicum ATCC 13032 to pH changes

Figure 3

Elution profile of membrane proteins in Anion-exchange Chromatography (AIEC). The washed and solubilized C. glutamicum membrane fractions at different pH conditions (1.2 mg protein each), were separated using AIEC as first dimension (1.5 ml column packed with PorosHQ20). Proteins were eluted by an increased salt gradient from 0.2 to 0.65 M NaCl in 38 column volumes (cv), followed by a sharp increase up to 1 M NaCl. The flow rate was set to 5 ml/min. Finally the column was washed with 6.5 cv of elution buffer and fractions of 1.5 ml were collected. A280 nm is indicated by red, blue and green lines for pH 6.0, pH 7.0 and pH 9.0 samples, respectively. The black line shows conductivity (salt concentration).

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