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Table 4 ELISA detection of rOmpL1-IgG/IgM in sera of leptospirosis patients

From: Characterization of the ompL1 gene of pathogenic Leptospira species in China and cross-immunogenicity of the OmpL1 protein

Sera

Cases (n)

Dilution (1:xxx)

IgG-ELISA positive (n)

IgM-ELISA positive (n)

   

rOmpL1/1

rOmpL1/2

rOmpL1/3

rOMPL1/1

rOMPL1/2

rOMPL1/3

Patients infected with:

        

Icterohaemorrhagia

191

50

/

/

/

145

161

139

  

100

159

187

149

132

152

128

  

200

147

162

135

/

/

/

Canicola

11

50

/

/

/

9

11

9

  

100

10

11

9

8

9

6

  

200

8

10

6

/

/

/

Autumnalis

13

50

/

/

/

13

11

9

  

100

13

12

10

10

8

7

  

200

12

9

7

/

/

/

Pomona

44

50

/

/

/

38

41

31

  

100

38

43

33

31

35

26

  

200

33

37

27

/

/

/

Australis

13

50

/

/

/

12

13

10

  

100

12

13

10

10

11

7

  

200

10

11

8

/

/

/

Grippotyphosa

31

50

/

/

/

29

26

22

  

100

30

26

23

23

21

16

  

200

26

23

16

/

/

/

Hebdomadis

37

50

/

/

/

34

35

30

  

100

33

36

30

29

30

25

  

200

28

31

24

/

/

/

Sejroe

45

50

/

/

/

41

37

36

  

100

43

38

35

37

32

28

  

200

37

33

29

/

/

/

Total

385

50

/

/

/

321

335

286

  

100

338

366

299

280

298

243

  

200

301

316

252

/

/

/

Healthy individuals:

36

50

/

/

/

0.20 ± 0.06

0.20 ± 0.07

0.19 ± 0.07

  

Cut-off

/

/

/

0.38

0.41

0.40

  

100

0.17 ± 0.05

0.18 ± 0.05

0.17 ± 0.06

0.18 ± 0.05

0.17 ± 0.06

0.17 ± 0.05

  

Cut-off

0.32

0.33

0.35

0.33

0.35

0.32

  

200

0.14 ± 0.04

0.13 ± 0.05

0.15 ± 0.04

/

/

/

  

Cut-off

0.26

0.28

0.27

/

/

/

  1. Note: the mean at OD490 + 3 SD = cut-off value