Figure 3

Initial adherence assays. A. Catheter adherence assay. Standardized overnight cultures of the wild type strain COL, the msa mutant and the complemented msa mutant were incubated with catheters at 37°C for 30 minutes. Results represent the mean ± SEM of three independent experiments. Student's paired t test was used to compare the msa mutant and the complemented msa mutant to the wild type strain (*denotes statistical significance of P < 0.05). B. Standardized overnight cultures of the wild type strain COL, the msa mutant and the complemented msa mutant were incubated for 1 hour at 37°C in plasma-coated microtitre wells. Adherent cells were then fixed with ethanol and then stained with Crystal Violet. Ethanol was then used to elute the wells and absorbance was measured. Results represent the mean ± SEM from three independent experiments.