From: Fluorescent-BOX-PCR for resolving bacterial genetic diversity, endemism and biogeography
Strain | DNA extractiona | PCRa | Gel/Injectiona | Number of observed fragment | RHb | RFc | ||||
---|---|---|---|---|---|---|---|---|---|---|
 |  |  |  | On agarose gel | On AbiPrism 310 |  |  | |||
 |  |  |  | Non fluorescent | HEX | 6-FAM | HEX | 6-FAM |  |  |
B. cereus BC360 | 1 | 1 | 1 | 7 | 1 | 1 | 34 | 36 | 4.9 | 5.1 |
 | 1 | 1 | 2 | 8 | n.d.e | n.d. | 34 | 36 | 4.3 | 4.5 |
 | 1 | 2 | 1 | 3 | 1 | 1 | 34 | 36 | 11.3 | 12.0 |
 | 1 | 2 | 2 | 3 | n.d. | n.d. | 34 | 36 | 11.3 | 12.0 |
 | 2 | 1 | 1 | 10 | 1 | 5 | 34 | 36 | 3.4 | 3.6 |
 | 2 | 1 | 2 | 7 | n.d. | n.d. | 34 | 36 | 4.9 | 5.1 |
 | 2 | 2 | 1 | 7 | 1 | 1 | 33 | 36 | 4.7 | 5.1 |
 | 2 | 2 | 2 | 5 | n.d. | n.d. | 33 | 36 | 6.6 | 7.2 |
CI1 23 | 1 | 1 | 1 | 13 | 8 | 11 | n.d. | 72 | n.a. | 5.5 |
 | 1 | 1 | 2 | 13 | n.d. | n.d. | n.d. | 72 | n.a. | 5.5 |
 | 1 | 2 | 1 | 16 | 6 | 10 | n.d. | 70 | n.a. | 4.4 |
 | 1 | 2 | 2 | 13 | n.d. | n.d. | n.d. | 70 | n.a. | 5.4 |
CI2 13 | 1 | 1 | 1 | 9 | 5 | 6 | 34 | 41 | 3.8 | 4.6 |
 | 1 | 1 | 2 | 9 | n.d. | n.d. | 34 | 41 | 3.8 | 4.6 |
 | 1 | 2 | 1 | 10 | 5 | 7 | 28 | 41 | 2.8 | 4.1 |
 | 1 | 2 | 2 | 9 | n.d. | n.d. | 29 | 41 | 3.2 | 4.6 |
 | 2 | 1 | 1 | 11 | 5 | 6 | 34 | 40 | 3.1 | 3.6 |
 | 2 | 1 | 2 | 11 | n.d. | n.d. | 33 | 40 | 3.0 | 3.6 |
 | 2 | 2 | 1 | 10 | 6 | 7 | 34 | 39 | 3.4 | 3.9 |
 | 2 | 2 | 2 | 11 | n.d. | n.d. | 34 | 40 | 3.1 | 3.6 |
CI2 17 | 1 | 1 | 1 | 10 | 7 | 10 | 26 | 50 | 2.6 | 5.0 |
 | 1 | 1 | 2 | 9 | n.d. | n.d. | 26 | 49 | 2.9 | 5.4 |
 | 1 | 2 | 1 | 18 | 4 | 10 | 27 | 52 | 1.5 | 2.9 |
 | 1 | 2 | 2 | 13 | n.d. | n.d. | 26 | 51 | 2.0 | 3.9 |
CI2 23 | 1 | 1 | 1 | 8 | 4 | 5 | 44 | 49 | 5.5 | 6.1 |
 | 1 | 1 | 2 | 12 | n.d. | n.d. | 44 | 49 | 3.7 | 4.1 |
 | 1 | 2 | 1 | 10 | 6 | 7 | 44 | 48 | 4.4 | 4.8 |
 | 1 | 2 | 2 | 11 | n.d. | n.d. | 44 | 48 | 4.0 | 4.4 |
 | 2 | 1 | 1 | 11 | 4 | 6 | 45 | 49 | 4.1 | 4.5 |
 | 2 | 1 | 2 | 11 | n.d. | n.d. | 45 | 49 | 4.1 | 4.5 |
 | 2 | 2 | 1 | 9 | 4 | 6 | 43 | 47 | 4.8 | 5.2 |
 | 2 | 2 | 2 | 12 | n.d. | n.d. | 44 | 47 | 3.7 | 3.9 |
CO2 33 | 1 | 1 | 1 | 12 | 3 | 5 | n.d. | 46 | n.a. | 3.8 |
 | 1 | 1 | 2 | 10 | n.d. | n.d. | n.d. | 46 | n.a. | 4.6 |
 | 1 | 2 | 1 | 9 | 3 | 5 | n.d. | 45 | n.a. | 5.0 |
 | 1 | 2 | 2 | 11 | n.d. | n.d. | n.d. | 45 | n.a. | 4.1 |
DS3 | 1 | 1 | 1 | 14 | 6 | 7 | 35 | 34 | 2.5 | 2.4 |
 | 1 | 1 | 2 | 13 | 6 | 6 | 35 | 34 | 2.7 | 2.6 |
 | 1 | 2 | 1 | 7 | 5 | 7 | 33 | 34 | 4.7 | 4.9 |
 | 1 | 2 | 2 | 6 | 4 | 5 | 33 | 34 | 5.5 | 5.7 |
 | 2 | 1 | 1 | 16 | 6 | 8 | 34 | 30 | 2.1 | 1.9 |
 | 2 | 1 | 2 | 13 | 6 | 7 | 33 | 31 | 2.5 | 2.4 |
 | 2 | 2 | 1 | 13 | 6 | 7 | 33 | 32 | 2.5 | 2.5 |
 | 2 | 2 | 2 | 8 | 6 | 6 | 33 | 32 | 4.1 | 4.0 |
E. coli DSM50902 | 1 | 1 | 1 | 19 | 10 | 13 | 26 | 57 | 1.4 | 3.0 |
 | 1 | 1 | 2 | 17 | n.d. | n.d. | 26 | 57 | 1.5 | 3.4 |
 | 1 | 2 | 1 | 18 | 3 | 9 | 24 | 57 | 1.3 | 3.2 |
 | 1 | 2 | 2 | 14 | n.d. | n.d. | 25 | 57 | 1.8 | 4.1 |
 | 2 | 1 | 1 | 19 | 8 | 14 | 26 | 58 | 1.4 | 3.1 |
 | 2 | 1 | 2 | 16 | n.d. | n.d. | 26 | 57 | 1.6 | 3.6 |
 | 2 | 2 | 1 | 15 | 9 | 15 | 26 | 60 | 1.7 | 4.0 |
 | 2 | 2 | 2 | 15 | n.d. | n.d. | 26 | 60 | 1.7 | 4.0 |