Figure 2From: Evaluation of an in silico predicted specific and immunogenic antigen from the OmcB protein for the serodiagnosis of Chlamydia trachomatis infectionsExpression and purification of the recombinant proteins and the vector tags. All proteins of interest are indicated with arrows. (A): Immunoblot analysis of the BPT tagged proteins expressed using the PinPoint Xa-1 vector in E. coli BL21 (DE3). Lane 1: molecular weight marker (broad range protein molecular marker, PROMEGA), lane 2: BPT (13 kDa), lane 3: BPT-Nt (24.5 kDa), lane 4: BPT-Ct (18.5 kDa). The star marks an endogenous biotinylated protein of 22.5 kDa expressed in E. coli cells. (B): SDS-PAGE (15%) analysis of GST tagged proteins expressed using the pGEX-6P-1 vector in E. coli BL21 (DE3). Lane 1: molecular weight marker, lane 2: GST (24 kDa), lane 3: GST-Nt (35.5 kDa), lane 4: GST-Ct (29.5 kDa). (C): SDS-PAGE (15%) analysis of the semi purified BPT-Ct by heat treatment. Lane 1: molecular weight marker, lane 2: 40°C 5 min, lane 3: 55°C 5 min, lane 4: 70°C 5 min. (D): SDS-PAGE (15%) analysis of purified GST and GST-Ct. Lane 1: molecular weight marker, lane 2: purified GST, lane 3: purified GST-Ct.Back to article page