Figure 1
Invasion of A. baumannii in epithelial cells. (A) NCI-H292 cells were infected with A. baumannii ATCC 19606T at an MOI of 100 up to 7 h. The colony-forming units were enumerated to measure the time-course of invasion. The result represents the mean ± standard deviation in duplicate wells and repeated a minimum of three separate times on separate days. (B) NCI-H292, HEp-2, and HeLa cells were infected with A. baumannii strains at an MOI of 100 for 5 h. (C) NCI-H292 cells were infected with A. baumannii 05KA103 at an MOI of 100 for 5 h. Actin was stained with Alexa Fluor ® 488 phalloidin (green). Bacteria were stained with polyclonal anti-rabbit AbOmpA antibody, followed by a secondary antibody Alexa Fluor ® 568 (red). The analytical sectioning was performed from the top to the bottom of the cells. The figure represents a single section of the cells. (D). NCI-H292 cells were infected with A. baumannii ATCC 19606T at an MOI of 100 for 5 h. Actin filaments have wrap-around-bacteria (white arrow). Red arrow indicates the extracellular bacteria. The figure represents all projection of sections in one picture.