Skip to main content

Table 2 Subcutaneous infection of G. mellonella

From: Insecticidal genes of Yersinia spp.: taxonomical distribution, contribution to toxicity towards Manduca sexta and Galleria mellonella, and evolution

    1:10 1:100 total  
strain tc -PAIYe total number dead alive dead alive dead alive dead [%] ± sd
Y. enterocolitica 2594 present2) 79 36 4 34 5 70 9 90 ± 9
Y. enterocolitica 4466 absent2) 96 47 1 34 14 81 15 88 ± 11
Y. mollaretii present1) 64 30 2 24 8 54 10 84 ± 5
Y. bercovieri absent1) 64 28 8 23 5 51 13 81 ± 11
Y. ruckeri absent2) 85 33 11 20 21 53 32 53 ± 21
W22703- tcaA::Tn 5 lux present, but tcaA knockout 93 30 25 18 20 48 45 51 ± 13
W22703 present 2) 114 32 25 20 37 52 62 41 ± 17
Y. aldovae absent2) 68 23 13 4 28 27 41 41 ± 6
Y. kristensenii absent2) 88 19 27 1 41 20 68 20 ± 12
controls          
S. typhimurium absent1) 88 10 33 10 35 20 68 18 ± 9
DH5α absent1) 63 5 34 2 22 7 56 13 ± 6
LB   64      3 61 5 ± 0
  1. G. mellonella larvae were subcutaneously infected with 5 μl of diluted overnight cultures. Strain W22703 and its tcaA knockout mutant confer similar toxicity towards the larvae (depicted in italics). Each experiment was independently performed at least three times, and the average dead rates are shown. Y. enterocolitica W22703: biotype 2, serotype O:9; Y. enterocolitica 2594: biotype 2, serotype O:9; Y. enterocolitica 4466: biotype 1B, serotype O:21. 1) according to the genome sequence, 2) according to PCR analysis performed in this study.