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Table 2 Subcutaneous infection of G. mellonella

From: Insecticidal genes of Yersinia spp.: taxonomical distribution, contribution to toxicity towards Manduca sexta and Galleria mellonella, and evolution

   

1:10

1:100

total

 

strain

tc -PAIYe

total number

dead

alive

dead

alive

dead

alive

dead [%] ± sd

Y. enterocolitica 2594

present2)

79

36

4

34

5

70

9

90 ± 9

Y. enterocolitica 4466

absent2)

96

47

1

34

14

81

15

88 ± 11

Y. mollaretii

present1)

64

30

2

24

8

54

10

84 ± 5

Y. bercovieri

absent1)

64

28

8

23

5

51

13

81 ± 11

Y. ruckeri

absent2)

85

33

11

20

21

53

32

53 ± 21

W22703- tcaA::Tn 5 lux

present, but tcaA knockout

93

30

25

18

20

48

45

51 ± 13

W22703

present 2)

114

32

25

20

37

52

62

41 ± 17

Y. aldovae

absent2)

68

23

13

4

28

27

41

41 ± 6

Y. kristensenii

absent2)

88

19

27

1

41

20

68

20 ± 12

controls

         

S. typhimurium

absent1)

88

10

33

10

35

20

68

18 ± 9

DH5α

absent1)

63

5

34

2

22

7

56

13 ± 6

LB

 

64

    

3

61

5 ± 0

  1. G. mellonella larvae were subcutaneously infected with 5 μl of diluted overnight cultures. Strain W22703 and its tcaA knockout mutant confer similar toxicity towards the larvae (depicted in italics). Each experiment was independently performed at least three times, and the average dead rates are shown. Y. enterocolitica W22703: biotype 2, serotype O:9; Y. enterocolitica 2594: biotype 2, serotype O:9; Y. enterocolitica 4466: biotype 1B, serotype O:21. 1) according to the genome sequence, 2) according to PCR analysis performed in this study.