Insecticidal genes of Yersinia spp.: taxonomical distribution, contribution to toxicity towards Manduca sexta and Galleria mellonella, and evolution

Table 1 Oral infection of M. sexta

strain	tc-PAI^Ye	total number	dead	alive	dead [%] ± sd
Y. enterocolitica 2594	present²⁾	18	15	3	83 ± 3
Y. mollaretii	present¹⁾	25	12	13	48 ± 9
Y. enterocolitica 4466	absent²⁾	36	15	21	42 ± 15
W22703	present²⁾	33	10	23	30 ± 14
Y. ruckeri	absent²⁾	19	4	15	21 ± 26
W22703-tcaA::Tn5 lux	present, but tcaA knockout	21	2	19	10 ± 11
Y. aldovae	absent²⁾	23	2	21	9 ± 2
control
DH5α	absent¹⁾	21	1	20	5 ± 7

Yersinia strains were orally fed to first-instar M. sexta neonates. Each experiment was independently performed at least three times with a minimum of six larvae, and the average dead rates are shown. Y. enterocolitica W22703: biotype 2, serotype O:9; Y. enterocolitica 2594: biotype 2, serotype O:9; Y. enterocolitica 4466: biotype 1B, serotype O:21. ¹⁾ according to the genome sequence, ²⁾ according to PCR analysis performed in this study.

ISSN: 1471-2180