Figure 6From: Uptake and intra-inclusion accumulation of exogenous immunoglobulin by Chlamydia-infected cellsIgG sequestration occurs in multiple cell lines. Non-phagocytic cells infected for 48 hpi were evaluated for the presence of bovine IgG within the chlamydial inclusion. Human epidermal HEp-2 (A), and mouse fibroblast McCoy (B) cell lines were evaluated by epi-fluorescence (1000×) and both were found to exhibit co-localization (yellow) of bovine IgG (detected with FITC-conjugated goat anti-bovine IgG, green) and the chlamydial inclusion (rabbit anti-Chlamydia/TRITC-conjugated goat anti-rabbit IgG, red), thus indicating that IgG has been translocated to the inclusion in these cell lines as well. (bar = 10 μm).Back to article page