Preventing abortion by inoculating with anti-Hsc70 antibody. (A) Number of live fetuses. Hsc70 was neutralized in the mice by administering with or without (control) an anti-mouse Hsc70 monoclonal antibody (clone R2–25)in vivo using 100 or 200 μg of the antibody. The control mice were given 100 μg of normal rat IgG. Statistically significant differences between the untreated control and antibody treated mice are indicated by asterisks (*, P < 0.01). (B) Bacterial numbers in spleen and placenta. On day 18.5 of gestation, the placenta and spleen were removed and homogenized in PBS. Tissue homogenates were serially diluted with PBS and plated on Brucella agar in order to count the number of CFU in each organ.