Bacterial internalization inhibiting antibodies react with Hsc70. (A) A membrane to which TG cell membrane fraction proteins had been transferred was stained with Coomassie brilliant blue (CBB) (lane 1), immunoblotted with the R2–25 antibody (lane 2) and the anti-Hsc70 antibody (clone SPA-815) (lane 3). (B) CBB staining of membrane to which mouse (M) or bovine (B) recombinant Hsc70 had been transferred. (C) Immunoblot analysis of mouse (M) or bovine (B) recombinant Hsc70 with R2–25 antibody and anti-Hsc70 antibody (clone SPA-815). (D) Amino acid sequences of Hsc70. The protein reacting with R2–25 was extracted from the PVDF membrane (square in lane 1 of panel A) and was used for LC-MS/MS analysis. The amino acids detected for Hsc70 in the analysis are shown in bold.