Table 2 Comparison between Y. pestis product sizes inferred by Agilent 2100 Bioanalyzer software (OBSERVED SIZE) and actual sizes obtained by direct sequencing of the PCR product or data available in Genbank (EXPECTED SIZE). UL BPS (unit length size).
MULTIPLEX | MARKER (final primer concentration, μM) | UL BPS | ALLELE | EXPECTED SIZE | OBSERVED SIZE | OFFSET |
|---|---|---|---|---|---|---|
1 | ypms51 (0.2) | 21 | 1 | 186 | 185 | 1 |
2 | 207 | 207 | 0 | |||
ypms01* (0.2) | 18 | 6 | 283 | 279 | 4 | |
7 | 301 | 291 | 10 | |||
9 | 337 | 329 | 8 | |||
10 | 355 | 344 | 11 | |||
2 | ypms04 (0.2) | 17 | 5 | 179 | 187 | -8 |
6 | 196 | 205 | -9 | |||
8 | 230 | 241 | -11 | |||
9 | 247 | 259 | -12 | |||
ypms62* (0.2) | 9 | 5 | 270 | 258 | 12 | |
6 | 279 | 267 | 12 | |||
9 | 306 | 285 | 21 | |||
10 | 315 | 294 | 21 | |||
12 | 333 | 308 | 25 | |||
15 | 360 | 331 | 29 | |||
20 | 405 | 366 | 39 | |||
3 | ypms07 (0.2) | 10 | 7 | 164 | 168 | -4 |
8 | 174 | 178 | -4 | |||
9 | 184 | 187 | -3 | |||
ypms05 (0.2) | 17 | 10 | 274 | 285 | -11 | |
11 | 291 | 300 | -9 | |||
4 | ypms74 (0.2) | 15 | 5 | 180 | 178 | 2 |
6 | 195 | 190 | 5 | |||
7 | 210 | 203 | 7 | |||
8 | 225 | 217 | 8 | |||
ypms15* (0.2) | 15 | 9 | 275 | 272 | 3 | |
10 | 290 | 284 | 6 | |||
5 | ypms35 (0.2) | 15 | 8 | 204 | 208 | -4 |
9 | 219 | 222 | -3 | |||
ypms40* (0.2) | 17 | 7 | 253 | 252 | 1 | |
8 | 270 | 266 | 4 | |||
9 | 287 | 277 | 10 | |||
6 | ypms38 (0.2) | 16 | 6 | 201 | 200 | 1 |
7 | 217 | 215 | 2 | |||
8 | 233 | 230 | 3 | |||
ypms20* (0.2) | 15 | 8 | 288 | 285 | 3 | |
9 | 303 | 300 | 3 | |||
7 | ypms41 (0.2) | 17 | 5 | 183 | 188 | -5 |
6 | 200 | 207 | -7 | |||
7 | 217 | 221 | -4 | |||
ypms56* (0.2) | 16 | 7 | 258 | 265 | -7 | |
9 | 290 | 296 | -6 | |||
8 | ypms45 (0.2) | 12 | 6 | 149 | 147 | 2 |
7 | 161 | 159 | 2 | |||
ypms44 (0.2) | 17 | 7 | 233 | 239 | -6 | |
8 | 250 | 260 | -10 | |||
ypms09* (0.2) | 18 | 22 | 732 | 671 | 61 | |
32 | 930 | 847 | 83 | |||
35 | 984 | 912 | 72 | |||
9 | ypms71 (0.2) | 14 | 5 | 157 | 163 | -6 |
6 | 171 | 178 | -7 | |||
ypms46 (0.2) | 7 | 3 | 238 | 243 | -5 | |
4 | 245 | 250 | -5 | |||
5 | 252 | 256 | -4 | |||
6 | 259 | 263 | -4 | |||
10 | 308 | 313 | -5 | |||
12 | 322 | 326 | -4 | |||
10 | ypms70 (0.2) | 9 | 5 | 137 | 145 | -8 |
6 | 146 | 155 | -9 | |||
8 | 164 | 174 | -10 | |||
9 | 173 | 185 | -12 | |||
11 | 191 | 205 | -14 | |||
ypms54 (0.2) | 22 | 6 | 259 | 265 | -6 | |
7 | 281 | 289 | -8 | |||
11 | ypms69 (0.2) | 16 | 5 | 163 | 164 | -1 |
6 | 179 | 177 | 2 | |||
7 | 195 | 195 | 0 | |||
ypms06 (0.2) | 60 | 3 | 306 | 309 | -3 | |
4 | 366 | 374 | -8 | |||
6 | 486 | 504 | -18 | |||
8 | 606 | 625 | -19 | |||
9 | 666 | 674 | -8 | |||
12 | ypms73 (0.2) | 18 | 5 | 207 | 212 | -5 |
6 | 225 | 231 | -6 | |||
ypms21* (0.2) | 18 | 9 | 329 | 339 | -10 | |
10 | 347 | 358 | -11 | |||
11 | 365 | 375 | -10 |