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Table 2 Comparison between Y. pestis product sizes inferred by Agilent 2100 Bioanalyzer software (OBSERVED SIZE) and actual sizes obtained by direct sequencing of the PCR product or data available in Genbank (EXPECTED SIZE). UL BPS (unit length size).

From: Fieldable genotyping of Bacillus anthracis and Yersinia pestis based on 25-loci Multi Locus VNTR Analysis

MULTIPLEX MARKER (final primer concentration, μM) UL BPS ALLELE EXPECTED SIZE OBSERVED SIZE OFFSET
1 ypms51 (0.2) 21 1 186 185 1
    2 207 207 0
  ypms01* (0.2) 18 6 283 279 4
    7 301 291 10
    9 337 329 8
    10 355 344 11
2 ypms04 (0.2) 17 5 179 187 -8
    6 196 205 -9
    8 230 241 -11
    9 247 259 -12
  ypms62* (0.2) 9 5 270 258 12
    6 279 267 12
    9 306 285 21
    10 315 294 21
    12 333 308 25
    15 360 331 29
    20 405 366 39
3 ypms07 (0.2) 10 7 164 168 -4
    8 174 178 -4
    9 184 187 -3
  ypms05 (0.2) 17 10 274 285 -11
    11 291 300 -9
4 ypms74 (0.2) 15 5 180 178 2
    6 195 190 5
    7 210 203 7
    8 225 217 8
  ypms15* (0.2) 15 9 275 272 3
    10 290 284 6
5 ypms35 (0.2) 15 8 204 208 -4
    9 219 222 -3
  ypms40* (0.2) 17 7 253 252 1
    8 270 266 4
    9 287 277 10
6 ypms38 (0.2) 16 6 201 200 1
    7 217 215 2
    8 233 230 3
  ypms20* (0.2) 15 8 288 285 3
    9 303 300 3
7 ypms41 (0.2) 17 5 183 188 -5
    6 200 207 -7
    7 217 221 -4
  ypms56* (0.2) 16 7 258 265 -7
    9 290 296 -6
8 ypms45 (0.2) 12 6 149 147 2
    7 161 159 2
  ypms44 (0.2) 17 7 233 239 -6
    8 250 260 -10
  ypms09* (0.2) 18 22 732 671 61
    32 930 847 83
    35 984 912 72
9 ypms71 (0.2) 14 5 157 163 -6
    6 171 178 -7
  ypms46 (0.2) 7 3 238 243 -5
    4 245 250 -5
    5 252 256 -4
    6 259 263 -4
    10 308 313 -5
    12 322 326 -4
10 ypms70 (0.2) 9 5 137 145 -8
    6 146 155 -9
    8 164 174 -10
    9 173 185 -12
    11 191 205 -14
  ypms54 (0.2) 22 6 259 265 -6
    7 281 289 -8
11 ypms69 (0.2) 16 5 163 164 -1
    6 179 177 2
    7 195 195 0
  ypms06 (0.2) 60 3 306 309 -3
    4 366 374 -8
    6 486 504 -18
    8 606 625 -19
    9 666 674 -8
12 ypms73 (0.2) 18 5 207 212 -5
    6 225 231 -6
  ypms21* (0.2) 18 9 329 339 -10
    10 347 358 -11
    11 365 375 -10
  1. * modified primer set (increased final fragment size)