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Figure 4 | BMC Microbiology

Figure 4

From: Functional characterization of a small heat shock protein from Mycobacterium leprae

Figure 4

(A). sHsp18 interacts with Sma I. Heat inactivated Sma I was added to, sHsp18 bound MagneHis particles as per methods. sHsp18 was able to interact and refold the heat inactivated Sma I and restore its biological activity. But in the control, heat inactivated Sma I without sHsp18 did not show any activity. Lanes represent, λ Hind III marker (lane 1), undigested plasmid (lane 2), plasmid DNA digested with heat inactivated Sma I added to sHsp18 bound MagneHis particles (lane 3), plasmid DNA treated with heat inactivated Sma I added to MagneHis particles (lane 4). (B) Sma I do not bind directly to MagneHis particles. Native Sma I was incubated with MagneHis particles for 5 min and the supernatant and pellet fractions were taken for restriction assay. Lanes represent, λ Hind III marker (lane 1). Plasmid DNA was digested with Sma I added MagneHis pellet fraction (lane 2), plasmid DNA digested with supernatant fraction (lane 3) and control digest (lane 4).

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