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Table 2 Survival of recombinant E. coli within THP-1 cells

From: Characterization of Mce4A protein of Mycobacterium tuberculosis: role in invasion and survival

Recombinant E. coli used to infect THP-1 cells

Colony forming units at different time points (% survival)

 

t0

t3

t24

Vector only

1426 ± 112

376 ± 37.74

(26.3 ± 1.8)

6.6 ± 1.45

(0.46 ± 0.12)

pET28a/mce4A

3157 ± 37.17

1493 ± 90.07

(47.2 ± 2.7φφ)

556 ± 60.80

(18.3 ± 2.17θθ)

pET28a/mce1A

3596 ± 106.4

2209 ± 146.3

(61.5 ± 4.59**)

770.7 ± 59.05

(21.33 ± 1.09***)

  1. Survival was assayed as colony forming units per milliliter of lysate following lysis of the infected cells. Time points considered were as follows: t0 - 1 hour, t3 - 3 hours and t24 - 24 hours, post-infection. All values were normalised to t0 value taken as 100%. The significance was calculated using t test. Recombinant E. coli expressing Mce4A or Mce1A survived inside THP-1 cells at a significantly high CFU as compared to recombinant E. coli with vector alone.
  2. φφ, p < 0.005; **, p < 0.005; when t test applied to compare percentage survival of E. coli (pET28a) with percentage survival of E. coli (pET28a/mce4A) and E. coli (pET28a/mce1A) respectively at 3 h of incubation.
  3. θθ, p < 0.005; ***, p < 0.0005; t test applied to compare percentage survival of E. coli (pET28a) with percentage survival of E. coli (pET28a/mce4A) and E. coli (pET28a/mce1A) respectively at 24 h of incubation.
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BMC Microbiology

ISSN: 1471-2180

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