Table 2 Oligo nucleotides.
From: Structural Prediction and Mutational Analysis of the Gifsy-1Xis Protein
Oligonucleotide | Sequence | Function |
|---|---|---|
Xis-1060f | TACCCACGCCGAAACAAG | Sequencing of pSM13-1 |
Xis genemorphf | ATTTCACACAGGAAACAGAATTCCCGGGGGA | PCR mutagenesis |
Xis Genemorphr | TCTCATCCGCCAAAACAGAAGCTTGGCTGCAGTTT | PCR mutagenesis |
pET27bplusXisNde1f | GCGCGCGCGCCATATGAGCAAAAAAATTAGAGACTTTGAATTGATG | Cloning of Gifsy-1 xis into pET27b+ |
pET27bplusXisBH1r | GCGCGCGCGCGGATCCTCACGCCACGTCAGACCGGATGAGTCGACC | Cloning of Gifsy-1 xis into pET27b+ |
T7 promoter | TAATACGACTCACTATAGGG | Sequencing of pET27b+ constructs |