Figure 5
From: Structural Prediction and Mutational Analysis of the Gifsy-1Xis Protein
MALDI-TOF analysis of Gifsy-1 Xis digested with proteinase K. Panel A contains a spectrum of undigested Gifsy-1 Xis. Panel B contains a spectrum of 20 minutes post addition of proteinase K. Panel C contains a spectrum 80 minutes post addition of proteinase K. Panel D contains a spectrum 160 minutes post addition of proteinase K. Peak identities are as follows. Peak 1: Residues 3–35, KKIRDFELMSTREICCQ LRISSRTLERYRKRPS; Peak 2: Residues 3–36, KKIRDFELMSTREICCQLRISSRTLERYRKRPSD; Peak 3: Residues 6–73, RDFELMSTREICCQLRISSR TLERYRKRPSDNNPFPEPDCSYMGGSNKWLKTKVNEWQVREMSRPTRR; Peak 4: Residues 2–72, SKKIRDFELMSTREICCQLRISSRTLERYRKRPSDNNPFPEPDCSYMGGSNK WLKTKVNEWQVRE MSRPTR; Peak 5: Residues 41–62, PEPDCSYMGGSNKWLKTKVNEW; Peak 6: Residues 9–30, ELMSTREICCQ LRISSRTLERY.