Isolation of the phe-operon from G. stearothermophilus comprising the phenol degradative meta-pathway genes and a novel transcriptional regulator

Table 2 Oligonucleotides for RT-PCR with the numbers indicating corresponding primer pairs

	Name	Sequence (5'-3')
1	pheB_down	TGA CGG AGT CAT TCA TCA AGG C
	pheA1_up	GCA AGA AGA GCA GCA GTG TCC AAC
2	pheA1_down	TTG CTA GCG GTG GCC GCA CAT CCT AAC
	pheA2_up	CGC ATT TGC AGT CAT GCC GTG TAT ATC C
3	pheA2_down	CAG GAA GAA GCC GAG CCA TTA ATC
	Fdx_up	CCT CGC CGC TAT CCA TTA CCT GCA C
4	Fdx_down	AGG AGG CGG ATG CGG AAT GTG CAA G
	pheR_up	AAG CAG CTG TCG GAA CCG TAA CC
5	pheR_down	CAA CCT TCC TAG CGT CAG TTA TC
	pheD_up	CCG CTT CTA TAA GGT GAT TAG C
6	pheD_down	GTG ATT CTT TCA GGC GCT CTT TCC G
	pheF_up	TTC CTG TGC TAA TGA CCG TAT ACC
7	pheF_down	ACG GAC TGA ACC AAT CAT GGA TGG A
	pheE_up	AGG AAC CGG CCA ATC CAT CTC CGT G
8	pheE_down	ATC GTA GCG CCA ATG CTT CCG GCA C
	pheC_up	CCT CTT GTA TCT TGT ATG CCT CTT CC
9	pheC_down	GTA TTC AAT CAC CCG GCC AAT TCT GTC G
	pheX_up	CCG CTT CTC CGC CAG AAG GAA GAT G

ISSN: 1471-2180