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Table 1 ORFs identified on a 20.2 kb fragment with the positions given relative to pheB, the predicted functions based on a similarity search and the molecular mass calculated from the deduced amino acid sequences

From: Isolation of the phe-operon from G. stearothermophilus comprising the phenol degradative meta-pathway genes and a novel transcriptional regulator

ORF Position [bp] Size [AS] Mass [kDa] Predicted Function
Rep -2211 – (-1053) 386 45.7 Plasmid replication initiator
pheB 1 – 912 304 34.7 Catechol 2,3-dioxygenase
pheH 951 – 1154 68 7.4 4-Oxalocrotonate tautomerase
pheA1 1340 – 2539 400 44.2 Phenol hydroxylase; Monooxygenase subunit
pheA2 2730 – 3184 152 16.9 Phenol hydroxylase; Flavin reductase subunit
fdx 3306 – 3707 134 14.9 Ferredoxin
pheR 3722 – 5647 642 74.3 Regulator
pheD 6011 – 6778 256 34.8 2-Oxopent-4-dienoate hydratase
pheF 6820 – 7701 294 36.7 Acetaldehyde dehydrogenase
pheE 7720 – 8745 342 36.7 4-Hydroxy-2-oxovalerate aldolase
pheC 8759 – 9559 267 28.9 4-Oxalocrotonate decarboxylase
pheX 9626 – 10303 226 25.8 Carboxylesterase/phospholipase
orfM 13269 – 12463 269 31.3 Unknown
orfP 14569 – 15129 187 21.5 Unknown
doc 15837 – 16256 140 16.5 Death-on-Curing; genetic addiction