Isolation of the phe-operon from G. stearothermophilus comprising the phenol degradative meta-pathway genes and a novel transcriptional regulator

Table 1 ORFs identified on a 20.2 kb fragment with the positions given relative to pheB, the predicted functions based on a similarity search and the molecular mass calculated from the deduced amino acid sequences

ORF	Position [bp]	Size [AS]	Mass [kDa]	Predicted Function
Rep	-2211 – (-1053)	386	45.7	Plasmid replication initiator
pheB	1 – 912	304	34.7	Catechol 2,3-dioxygenase
pheH	951 – 1154	68	7.4	4-Oxalocrotonate tautomerase
pheA1	1340 – 2539	400	44.2	Phenol hydroxylase; Monooxygenase subunit
pheA2	2730 – 3184	152	16.9	Phenol hydroxylase; Flavin reductase subunit
fdx	3306 – 3707	134	14.9	Ferredoxin
pheR	3722 – 5647	642	74.3	Regulator
pheD	6011 – 6778	256	34.8	2-Oxopent-4-dienoate hydratase
pheF	6820 – 7701	294	36.7	Acetaldehyde dehydrogenase
pheE	7720 – 8745	342	36.7	4-Hydroxy-2-oxovalerate aldolase
pheC	8759 – 9559	267	28.9	4-Oxalocrotonate decarboxylase
pheX	9626 – 10303	226	25.8	Carboxylesterase/phospholipase
orfM	13269 – 12463	269	31.3	Unknown
orfP	14569 – 15129	187	21.5	Unknown
doc	15837 – 16256	140	16.5	Death-on-Curing; genetic addiction

ISSN: 1471-2180