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Figure 5 | BMC Microbiology

Figure 5

From: Isolation of the phe-operon from G. stearothermophilus comprising the phenol degradative meta-pathway genes and a novel transcriptional regulator

Figure 5

Result of RT-PCR. The intergenic spaces were amplified from a cDNA template to prove that the phe-genes including fdx are transcribed in one polycistronic mRNA. Lane 1, intergenic space pheB/pheA1 (including pheH)-expected size 512 bp; 2, pheA1/pheA2-365 bp; 3, pheA2/fdx-333 bp; 4, fdx/pheR-298 bp; 5, pheR/pheD-515 bp; 6, pheD/pheF-318 bp; 7, pheF/pheE-353 bp; 8, pheE/pheC-395 bp; 9, pheC/pheX-310 bp. The lane numbers also refer to the respective primer pairs listed in table 2 used for the amplification.

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BMC Microbiology

ISSN: 1471-2180

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