A short-oligonucleotide microarray that allows improved detection of gastrointestinal tract microbial communities

BMC Microbiology

Table 3 Microarray binding levels of selected 16–21-mer microarray probes from healthy individuals and a UC patient

Microarray Probe	Individual A	Individual B	Individual C	Disease	Remission
B. longum grp1*	+++	+	+	++++++	+++
B. longum grp2*	++++	+	+	+++	++
B. longum grp3*	++++++	++	+	++++++	++++++
B. bifidium*	++++++	+	+	+	+
B. thetaiotaomicron & B. ovatus	++++++	++++	++++++	+	+++++
B. ovatus	+	+	+	-	+
B. thetaiotaomicron	++	+	+++	-	-
B. vulgatus1	++++++	+++	++++	+	++++++
B. vulgatus2	+	+	+	-	-
B. putredinis1	++++++	+	+	+	++
B. putredinis2	++++	+	+	-	+
D. piger2	-	-	-	-	+
D. piger4	-	-	-	+	++
Enterobacteriaceae1*	++++	++++++	++++++	++	+
Enterobacteriaceae2	-	+	+	-	-
CclusterXIVab*	++++++	++++	++++++	++	++
C. coccoides	++++++	++++++	++++++	++++	++
C. clostridiformes1	+++	+	++	+	++
C. nexile	+	+	+	-	-
C. symbiosum	+	+	+	-	-
CclusterIV	++	+	+	+	-
C. leptum2*	+	+	+	+	+
C. butyricium	+	+	+	-	-
C. paraputrificum	+	-	+	-	-
ClusterIII	++++++	++++++	+++	++	+
E. cylindroides clust1	++++++	++++	++++	+	+
E. cylindroides clust2	++++++	+++++	+++++	+++	+
E. biforme3	+	+	+	++	+
E. biforme4	+	+	+	+	+
E. rectale1	++	+	+	++	+
E. rectale2	+++++	+	++	+++	+
R. lactaris	++++	+	+	+	+
R. torques	+	-	-	-	-
R. albus & R.flavefaciens1	++	+	+	-	-
R. albus & R. flavefaciens2	++++++	++++	++	++	+
R. callidus	+	+	++	-	-
R. bromii	++++++	++	+++	-	-
Verrucomicrobiales	++	++++++	++++++	-	-

Microarray binding levels of selected 16–21-mer microarray probes from three healthy individuals and a UC sufferer during active disease and remission phases.
Hybridisation intensities, converted into symbols are: no signal to 0.09, -; 0.10 to 0.99, +; 1.00 to 1.99, ++; 2.00 to 2.99, +++; 3.00 to 3.99, ++++; 4.00 to 4.99, +++++; 5.00 or greater, ++++++. Probes highlighted with a * indicate they were used for initial specificity testing.

ISSN: 1471-2180