A short-oligonucleotide microarray that allows improved detection of gastrointestinal tract microbial communities

BMC Microbiology

Table 2 16S ribosomal gene primers used for PCR amplification or DGGE

Primer	Sequence (5'-3')	Primer target site^a	Reference
Amp F	GAGAGTTTGATYCTGGCTCAG	0006	[13]
Amp R	AAGGAGGTGATCCARCCGCA	1530	[13]
InfL6^b	TATCGGGGAGCAAGCGAG	0445	[53]
Bif662-r	CCACCGTTACACCGGGAA	0662	[3]
ENT183^c	CGTCGCAAGACCAAAGAG	0183	[54]
424R	CCGCTGAAAGTACTTTACAACC	0424	This study
F-968^d	GAACGCGAAGAACCTTAC	0968	[55]
R-1401	CGGTGTGTAGAAGACCC	1401	[55]
F-341^d	CCTACGGGAGGCAGCAG	0341	[56]
R-534	ATTACCGCGGCTGCTGG	0534	[56]

^aPrimer target site according to E. coli 16S ribosomal gene numbering as the reference.
^bThis primer was known as B. longum grp3 for the microarray.
^cThis primer was designated Enterobacteriaceae1 for the microarray.
^dA 39 nt GC clamp was added to the 5' end of the primer when used for DGGE.

ISSN: 1471-2180