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Figure 6 | BMC Microbiology

Figure 6

From: Expression of recombinant Clostridium difficile toxin A and B in Bacillus megaterium

Figure 6

Disruption of tight junctions by recombinant toxins. HCT-8 cells on transwells were cultured untill the formation of tight junctions. The polarized monolayers were untreated (A) or treated with 300 ng/ml of rTcdA for 2 hr (B), 4 hr (C), and 6 hr (D); or with rTcdB 300 ng/ml for 2 hr (E), and 4 hr (F). Cells on the transwell membrane were fixed and stained with anti-oocludin and fluorochrome-conjugated secondary antibodies, and then visualized under a confocal microscope.

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