Confocal microscopy images of dual labelled immunofluorescence of HtrA and LPS during C. trachomatis L2 HEp-2 cultures. The figure shows immunocytochemistry of acute, penicillin persistent, and IFN-γ persistent cultures fixed and stained with HtrA and LPS antibodies at 20 h and 44 h post infection. The LPS antibody (shown in green, column 1) has a FITC label directly conjugated to the antibody, the HtrA antibody (shown in red, second column) was detected using a secondary antiRabbit IgG antibody with an Alexa Fluor label (561). Overlays are shown in the third column. Scale bars (10 μM) are shown in the bottom right of each figure.