Figure 4

SDS-PAGE of total extracellular proteins of MFN032 and the PlcC mutants MFN1037 and MFN1038. Supernatants of cultures at 17°C in LB medium were concentrated on an Amicon ultra-15 filter and subjected to SDS-PAGE in a 10% acrylamide gel. The gel was silver-stained (lanes 1 to 3) and placed against an egg-yolk agar plate (lanes 4 to 6) to reveal lecithinase activity (the opaque band on the plate). 1 and 6: MFN1032; 2 and 5: MFN1037 (plcC-deficient MFN1032); 3 and 4: MFN1038 (plcC complemented MFN1037). M- molecular size markers. a: PlcC; b: flagellin.