Figure 2

Effect of growth temperature on the cytotoxicity of MFN1032 (white square), MF37 (grey square), and PAO1 (black square). After 15 generations in LB medium, aliquots of cell-free supernatants were assayed for glial cell lysis (A) or hemolysis (B), as described in the materials and methods. For assays of glial cell cytotoxicity, supernatants were concentrated with an Amicon ultra-15 filter, and then resuspended in glial cell medium. Each experiment was performed at least three times in triplicate. Nd: not determined.