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Table 1 Bacterial strains and plasmids used in this study

From: Functional analysis of the group A streptococcal luxS/AI-2 system in metabolism, adaptation to stress and interaction with host cells

Strain/plasmid

Relevant characteristics

Source

Streptococcus pyogenes

   RDN29

M1 type

ATCC 700294

   RDN02

M19 type

Lab strain collection

   EC478

Isogenic luxS-deficient mutant of RDN29

This study

   RDN306

Isogenic luxS-deficient mutant of RDN02

This study

   EC460

RDN29 (pEC82)

This study

   EC489

EC478 (pEC82)

This study

   EC490

EC478 (pEC83)

This study

   EC480

RDN02 (pEC82)

This study

   EC481

RDN306 (pEC82)

This study

   EC482

RDN306 (pEC83)

This study

Escherichia coli

   DH5α

Host for cloning; AI-2 deficient (contains a frame-shift mutation in luxS)

Lab strain collection

   TOP10

Host for cloning

Invitrogen

   EC467

DH5α (pEC82)

This study

   EC464

DH5α (pEC83)

This study

Vibrio harveyi

   BB170

AI-1 sensor deficient, AI-2 sensor positive

[9]

Plasmids

   pAT21

Apr Kmr; pBR322Ω 1.5-kb pJH1 Cla I (aphIII)

[61]

   pUC19

ColE1ori, Ampr, lacZ

New England Biolabs

   pEC131

pUC19ΩluxS up-aphIII-luxS down

This study

   pEC82

repDEG-pAMβ1, ermAM/B, ColE1ori

This study

   pEC83

pEC82ΩPluxS-luxS-TT

This study