Strain/plasmid | Relevant characteristics | Source |
---|---|---|
Streptococcus pyogenes | ||
   RDN29 | M1 type | ATCC 700294 |
   RDN02 | M19 type | Lab strain collection |
   EC478 | Isogenic luxS-deficient mutant of RDN29 | This study |
   RDN306 | Isogenic luxS-deficient mutant of RDN02 | This study |
   EC460 | RDN29 (pEC82) | This study |
   EC489 | EC478 (pEC82) | This study |
   EC490 | EC478 (pEC83) | This study |
   EC480 | RDN02 (pEC82) | This study |
   EC481 | RDN306 (pEC82) | This study |
   EC482 | RDN306 (pEC83) | This study |
Escherichia coli | ||
   DH5α | Host for cloning; AI-2 deficient (contains a frame-shift mutation in luxS) | Lab strain collection |
   TOP10 | Host for cloning | Invitrogen |
   EC467 | DH5α (pEC82) | This study |
   EC464 | DH5α (pEC83) | This study |
Vibrio harveyi | ||
   BB170 | AI-1 sensor deficient, AI-2 sensor positive | [9] |
Plasmids | ||
   pAT21 | Apr Kmr; pBR322Ω 1.5-kb pJH1 Cla I (aphIII) | [61] |
   pUC19 | ColE1ori, Ampr, lacZ | New England Biolabs |
   pEC131 | pUC19ΩluxS up-aphIII-luxS down | This study |
   pEC82 | repDEG-pAMβ1, ermAM/B, ColE1ori | This study |
   pEC83 | pEC82ΩPluxS-luxS-TT | This study |