Phenotypic screening, transcriptional profiling, and comparative genomic analysis of an invasive and non-invasive strain of Candida albicans

Table 1 Growth conditions used for phenotypic characterisation of C. albicans strains SC5314 and ATCC10231

Condition tested	Medium/Supplementation^a
Control	SD and YPD without any supplementation
Carbon source^b	galactose, glycerol, mannitol, ethanol^c (2% (w/v) each)
pH value	pH 4, pH 5, pH 6, pH 7, pH 8
Temperature	18°C, 30°C, 37°C, 42°C
Elevated cation concentration	NaCl (1 M and 1.3 M), CaCl₂ (50 mM and 300 mM)
Anaerobic growth	anaerobic jar, embedded conditions (YPS [78])
Hyphae induction	10% foetal calf serum, Lee's medium [79], Spider medium [80], GlcNac, embedded (YPS), SLAD [81]
Resitance towards antimycotics	hygromycin B (400 μg/ml), amorolfin (3 μg/ml), itraconazole (1 μg/ml), caspofungin (200 ng/ml)
Stress	calcofluor white (800 μg/ml), benomyl (200 μg/ml), congo red (100 μg/ml and 150 μg/ml), cyclosporin A (5 mM and 10 mM), NaF (30 mM and 40 mM), 5-FOA (0.05% (w/v)), caffeine (20 mM), SDS (0.01% (w/v))
Extracellular enzyme activity	BSA agar, egg yolk agar

^aIf not otherwise indicated, the basic medium was SD and media were incubated at 30°C and 37°C.
^bTo test the ability for utilizing different carbon sources, the glucose in the SD medium was exchanged with the same amount of the indicated carbon source.
^cSupplements in bold are those that showed significant different growth of the two strains.

ISSN: 1471-2180