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Table 1 Growth conditions used for phenotypic characterisation of C. albicans strains SC5314 and ATCC10231

From: Phenotypic screening, transcriptional profiling, and comparative genomic analysis of an invasive and non-invasive strain of Candida albicans

Condition tested Medium/Supplementationa
Control SD and YPD without any supplementation
Carbon sourceb galactose, glycerol, mannitol, ethanolc (2% (w/v) each)
pH value pH 4, pH 5, pH 6, pH 7, pH 8
Temperature 18°C, 30°C, 37°C, 42°C
Elevated cation concentration NaCl (1 M and 1.3 M), CaCl2 (50 mM and 300 mM)
Anaerobic growth anaerobic jar, embedded conditions (YPS [78])
Hyphae induction 10% foetal calf serum, Lee's medium [79], Spider medium [80], GlcNac, embedded (YPS), SLAD [81]
Resitance towards antimycotics hygromycin B (400 μg/ml), amorolfin (3 μg/ml), itraconazole (1 μg/ml), caspofungin (200 ng/ml)
Stress calcofluor white (800 μg/ml), benomyl (200 μg/ml), congo red (100 μg/ml and 150 μg/ml), cyclosporin A (5 mM and 10 mM), NaF (30 mM and 40 mM), 5-FOA (0.05% (w/v)), caffeine (20 mM), SDS (0.01% (w/v))
Extracellular enzyme activity BSA agar, egg yolk agar
  1. aIf not otherwise indicated, the basic medium was SD and media were incubated at 30°C and 37°C.
  2. bTo test the ability for utilizing different carbon sources, the glucose in the SD medium was exchanged with the same amount of the indicated carbon source.
  3. cSupplements in bold are those that showed significant different growth of the two strains.