Plot of the signal ratio and the sequence homology (compared to strain SC5314) of different genes from strain ATCC10231. Thirty-seven genes gave at least a 2-fold weaker signal with DNA from strain ATCC10231 compared to SC5314 (filled circles). Additionally, 15 genes were included which were already sequenced and published (open circles). Filled triangles indicate 5 genes that gave a stronger signal with DNA from ATCC10231 compared to SC5314. The cut-off value for substantial differences within the sequence homology of the two strains was set at 90%. Only three genes (PHO81, HAL21, HAL22) fulfilled the criteria of both low signal ratio and low sequence homology.