Table 3 Primers used in this study
From: The YvfTU Two-component System is involved in plcR expression in Bacillus cereus
Primer name | 5'-3' sequencea | Restriction sites |
|---|---|---|
5Up-KR | GCTACCATGGCTTCTAAAAATTACACCGCTTC | NcoI |
3Up-KR | GCTAGTCGACCATTAAAATAAACATTGGAACG | SalI |
5Dn-KR | GCTACTGCAGAGATTTAATGTTTGGCTTATGG | PstI |
3Dn-KR | GGTAGGATCCTACACGGTTTTGATTCTACTGA | BamHI |
Km5in | TCTGGTCGACCATTTGAGGTGATAGG | SalI |
Km3in | GCTACTGCAGATCGATACAAATTCCTCGTAGGCG | PstI |
Km5out | CGGTATAATCTTACCTATCACC | |
Km3out | TACTCTGATGTTTTATATCTTTTCTAA | |
PyvfT-F | CGCCTGCAGTTATTAATGCCTGTTATGTTTT | PstI |
PyvfT-R | CGCGGATCCTGTCTTTTTCTTATATATATTG | BamHI |
Cp-yvfTU | CGTCGAATTCATTTGAAGCACACGGTTAC | EcoRI |
PplcR-R | GGCGTCTAGACCCATTAGAACAATCTAATTTT | XbaI |
PplcR-F | GACGAAGCTTATTCATTTGATACGGCAGTG | HindIII |
5355-F | GATAGTATTACGGTGGAAGAGG | |
5355-R | AAACTGTTCAAATGCTTCAT | |
5355-54-R | CTGTTTGAGCGATAATTTTT | |
5354-R | CTGTTTGAGCGATAATTTTT | |
yvfT-54-R | CTATTACAAGCTTCCATCCTGATGCC | |
yvfTU-F | TTGTGAAAAATCCAGAGCGTGC | |
yvfTU-R | ATCCAATCCACTTTGAATCGGC | |
yvfT-R2 | CTATTACAAGCTTCCATCCTGATGCC | |
yvfT-R3 | CGCAAGCTTCAAACCACATATACGGGAAG | |
LC-16S-F | GGTAGTCCACGCCGTAAACG | |
LC-16S-R | GACAACCATGCACCACCTG | |
LC-plcR-F | TCCAGCAATTTCTTCAATGG | |
LC-plcR-R | TCGGCATGATATTTCAATCG | |
LC-hblC-F | TTCAAGCAGAAACTCAACAG | |
LC-hblC-R | TTCAAGTCTATCCGAAAACC |