Figure 5

2D-GE of WT and yvfTU mutant culture supernatants collected 2 hours after the onset of stationary phase. Spots found in lower amount in the extracellular proteome of the yvfTU mutant correspond to NprB and PC-PLC proteins, as confirmed by Mass Spectrometry identification. The different spots correspond to isoforms of the same protein, with distinct charges but identical molecular weights. The gel areas shown are located around 37 kDa with a pI between 5.5 and 6 for NprB, and 25 kDa with a pI between 6.0 and 7.0 for PC-PLC.