Figure 2

RT-PCR detection of yvfTU , BC5354 and BC5355 in B. cereus strain ATCC 14579. Lane "+": Positive control (PCR on genomic DNA). Lanes "RT": RT-PCR on 500 ng RNA. Lanes "-": Negative control (RT-PCR on 500 ng RNA with a heat-inactivated reverse-transcriptase). Numbers in brackets refer to the positions of the RT-PCR products on the yvfTU locus, as represented on Fig. 1A. RNA extraction was performed on strains grown at 37°C in LB broth and harvested in exponential phase (E) (OD₆₀₀ 1), or 2 hours after the onset of stationary phase (T2).