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Table 1 Strains and plasmids used in this study.

From: Acid stress damage of DNA is prevented by Dps binding in Escherichia coliO157:H7

Strains and plasmid

Relevant characteristicsa

Reference or source

Strains

  

DH5α

supE44 ΔlacU169 (Φ80 LacZ ΔM15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1

Lab collection

SY327 λpir

Δ (lac operon) argE(Am) recA56 rpoB λpir; host for π-requiring plasmids

[26]

SM0 λpir

thi thr leu tonA lacY supE recA::RP4-2-Tc::Mu λpir, oriT of RP4, Kmr

[38]

BL21(DE3)

BL21λ (DE3) under lac control; ompT lon dcm

Novagen

ATCC43895

stxI stxII serotype O157:H7

Lab collection

FRIK47992

ATCC43895, dps::npt1

[10]

FRIK4704-kcj05

ATCC43895, ΔrecA

This study

FRIK4704-kcj06

FRIK47992 (ATCC43895, dps, ΔrecA)

This study

Plasmids

  

pUC4K

pUC4 with nptI; Apr, Kmr

[39]

pCVD442

R6K γori, sacB, oriT of RP4; Apr

[24]

pKCJ0325

pET21b with dps; Apr

This study

pKCJ0328

pCVD442 with alas::mltB; Apr

This study

  1. aApr, ampicillin resistant; Kmr, kanamycin resistant