Transcriptional regulation of subclass 5b fimbriae

BMC Microbiology

Table 1 Activation of fimbrial promoters by Rns

	Mean β-galactosidase activity (Miller Units) ± SD^a
Reporter	rns::kan	rns+	fold activation
CS17p::lacZYA	89 ± 3	718 ± 163	8
CS19p::lacZYA	487 ± 94	4670 ± 176	10
PCFO71p::lacZYA	144 ± 32	1462 ± 139	10

Reporter strains were grown aerobically at 37°C in LB medium with 100 μg/ml ampicillin and harvested from the late log phase cultures with an optical absorbance of 0.4 to 0.6 at 580 nm. ^aSD, standard deviation; n ≥ 3.

ISSN: 1471-2180