Figure 2

Identification of Rns binding sites in vitro. DNase I footprints of MBP–Rns bound the noncoding strands of CS17, CS19, and PCFO71 fimbrial promoters. The nucleotide sequence of each promoter is shown below each gel image with predicted -10 and -35 hexamers in boxes. Numbering is relative to the Rns–dependent transcription start site, denoted by wavy arrows, of each promoter. The central 12 nucleotides of each binding site, which are partially conserved, are shown in bold. Lanes labeled TC and GA contain Maxam–Gilbert sequencing ladders.