PCR and real-time PCR primers developed for detection and identification of Bifidobacterium thermophilumin faeces

Table 1 Oligonucleotides used in this study

Oligonucleotide	Sequence 5'-3'	Reference
btherm	GAT GTG CCG GGC TCC TGC ATG	This study
lm3	CGG GTG CTI CCC ACT TTC ATG	[20]
lm26	GAT TCT GGC TCA GGA TGA ACG	[20]
bak11w	AGT TTG ATC MTG GCT CAG	[21]
bak4	AGG AGG TGA TCC ARC CGC A	[22]
bthermRTF	TTG CTT GCG GGT GAG AGT	This study
bthermRTR	CGC CAA CAA GCT GAT AGG AC	This study
bthermTqM	FAM-ATG TGC CGG GCT CCT GCA T-TAMRA	This study
520F	CAG GAG TGC CAG CAG CCG CGG	[23]
520R	ACC GCG GCT GCT GGC	[23]
1100F	CAG GAG CAA CGA GCG CAA CCC	[23]
1100R	AGG GTT GCG CTC GTT G	[23]

*FAM (6-carboxyfluorescein): fluorescent reporter dye. TAMRA (6-carboxytetramethylrhodamine): quencher.

ISSN: 1471-2180