Figure 4From: PCR and real-time PCR primers developed for detection and identification of Bifidobacterium thermophilumin faecesDetection of B. thermophilum in faecal samples by qPCR targeting the 16S rDNA. Cycle threshold (CT) values measured for faecal samples from 13 children (C1 to C13) and 4 adults (A1 to A4). F0: PCR-negative faecal sample, w: water instead of DNA, RBL67: DNA from pure culture of RBL67, 21395: DNA from B. thermacidophilum subsp. thermacidophilum LMG21395T. Values are means and standard deviations for three repetitions of the qPCR assay with three replicates each.Back to article page