Figure 3From: PCR and real-time PCR primers developed for detection and identification of Bifidobacterium thermophilumin faecesDetermination of the detection limit of the B. thermophilum PCR on faecal samples. PCR amplification with primers btherm and lm3 of DNA isolated from faecal sample F0, the PCR-amplification negative control (0) spiked with 109 (9), 108 (8), 107 (7), 106 (6) or 105 (5) B. thermophilum RBL67 cells/g faeces; h: 100-bp DNA ladder [kb]; t: 1-kb DNA ladder [kb]; w: water; 67: PCR on a colony of B. thermophilum RBL67.Back to article page