Figure 2
Specificity of the PCR for B. thermophilum using primers btherm and lm3. Agarose gel electrophoresis of PCR products (1.3 kb) obtained with primers btherm/lm3 on colonies of B. thermophilum RBL67 (1), B. thermacidophilum subsp. porcinum RBL68 (2), B. thermacidophilum subsp. porcinum RBL70 (3), B. thermacidophilum subsp. thermacidophilum LMG21395T (4), B. thermacidophilum subsp. porcinum LMG21689T (5), B. thermophilum DSM20210T (6), B. boum DSM20432T (7), B. breve DSM20213T (8), Bifidobacterium longum NCC2705 (9), Bifidobacterium coryneforme DSM2026T (10), Bifidobacterium asteroides DSM20089T (11), Bifidobacterium animalis subsp.lactis DSM10140 (12), B. animalis subsp. animalis DSM20105 (13), Bifidobacterium cuniculi DSM20435T (14), Bifidobacterium adolescentis DSM20083T (15), Bifidobacterium bifidum DSM20456T (16), Lactobacillus delbrueckii subsp. lactis DSM20072T (17) and Lactobacillus plantarum subsp.plantarum DSM20174T (18), t: Tridye 1-kb DNA ladder, in kb (New England Biolabs, Ipswich, MA, USA).