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Table 3 Allelotyping PCR scheme for presumptive identification of S. enterica serovars Enteritidis, Hadar, Heidelberg, and Typhimurium

From: Rapid screening of Salmonella entericaserovars Enteritidis, Hadar, Heidelberg and Typhimurium using a serologically-correlative allelotyping PCR targeting the O and H antigen alleles

O-multiplex H1-multiplexes1 H2-multiplex Serovars Sensitivity5 Specificity5
B i I2 Typhimurium 1.00 1.00
B r I Heidelberg 1.00 1.00
C2 z10 II3 Hadar 1.00 1.00
A/D1 g,m -4 Enteritidis 1.00 0.96
  1. 1Identifies H1 alleles i; g,m; r; or z10
  2. 2 Covers H2 alleles 1,2; 1,5; 1,6; and 1,7
  3. 3 Covers H2 alleles e,n,x and e,n,z15
  4. 4 PCR negative for H2-multiplex
  5. 5Sensitivity and specificity of the allelotyping PCR scheme relative to conventional serotyping in identifying S. enterica serovars Enteritidis, Hadar, Heidelberg, and Typhimurium among the 239 isolates examined in this study.